Research Article
Heat Shock Induced Triple Helix DNA and it’s Refolding to Duplex by HSP70 in Yeast
Pasha SM1, Musfera S2, Hari Narayana Kola1,3, Rao LV1 and Pasha C1*
1Department of Microbiology, Nizam College, Osmania University, Hyderabad, India
2Department of Microbiology, Shantinikethan Women’s College, Osmania University, Hyderabad, India
3Department of Biotechnology, Vignan’s Foundation For Science Technology & Research, Deemed to be University, Vadlamudi,
Guntur District, Andhra Pradesh, India
*Corresponding author: Pasha C, Department of Microbiology, Nizam College, Osmania University, Hyderabad, India; E-mail:
cpasha21@gmail.com
Copyright: © 2023 Pasha SM, et al. This is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Article Information: Submission: 10/12/2022; Accepted: 11/01/2023; Published: 16/01/2023
Abstract
Heat can damage and inhibit the synthesis and repair of damage to DNA, proteins, RNA. Heat shock increases DNA damage by crosslinking and
breakages. Crosslinking creates the multi strand DNA, which down regulates many genes. Triple helix is a major form of multi strand DNA reported to exist.
Using poly purine templates for BLAST, number of possible triple helical structures were predicted. Frame shift assays using denaturing PAGE, UV spectra,
melting complexes, DMSO foot printing and RNAse T treatment were analyzed for triple helix formation. Heat shock caused accumulation of triple helix DNA
in yeast. Heat shock proteins were reported and found to bind the triple helical DNA. Double stranded DNA structure was retrieved from triple helix with the
help of HSP 70. This study confirms the possible role of HSP 70 for refolding of misfolded DNA like that of proteins.