Metabolic engineers are examining the non food oil seed to develop suitable crop with enhanced performance in fatty acid functionalities and properties. The growth and production of various innovative fatty acids find various application for industrial processes. Crambe species of perennial flowering plants have miniscule white and yellow flowers. These are primarily used as food plants by larvae of some Lepidoptera class. Crambe is a unique seed crop exclusive grown for its industrial manufacturing especially enhanced oleic acid content by the Crambe genes of Fatty Acid Desaturase (FAD) and Fatty Acid Elongase (FAE) via down-regulated process. The FAD 2 enzymes are expressed in different manner in various tissues of the plants and transforms the functional and vegetative features due over expression. This study involves the computational analysis of the Crambe oleate desaturase and fatty acid elongase1 genes using various web tools with the ultimate aim of providing recommendations to wet lab researchers. The oleate desaturase and fatty acid elongase1 (FAE 1) sequences from Crambe were retrieved from NCBI nucleotide database. Lipases can be tailored to mitigate the carbon chain length, extent of unsaturation, and relevant improvement in the esterification reaction. This is significantly important for molecular pathway in delta-12 desaturases FAD2. These studies will be highly beneficial in predicting the feasibility of generating amplified wax ester oils in transgenic crops, fatty acid reshaping such as oleyl-oleate for the availability of enhanced levels of fatty acid formation in vegetative tissues. The study will be useful for the better understanding of the demonstration, regulation and mechanism that will help in enhancing the oil seed characteristics and confrontation towards various temperature and salt stresses in plants. These studies will be quite useful for the necessity of timely desirable traits in vegetable oil produce with reference to carbon economy, to meet the demand of traditional fossil fuel advancement and human health.