Elettaria cardamomum seeds stores robust phytochemicals responsible for several therapeutic efficacy, hence it is a rich source of useful components that has medicinal properties and one of the main applications is in controlling bleeding. Thus, current study investigates the antioxidant, procoagulant and clot fibrinolytic efficiency of Elettaria cardamomum Seed Aqueous Extract (ECSAE). The antioxidant potential of the extract was assessed by antioxidant activity using 2, 2 - diphenyl, 1- picryl hydrazyl (DPPH) radical scavenging activity. The ECSAE was found to have 63.00% of DPPH scavenging activity with an IC50 value of 45.48μg/ml compare to positive control ascorbic acid. Preliminary phytochemical screening was analyzed by instrumentation method such as RP-HPLC and GC-MS. The protein blue prints of ECSAE revealed similar banding pattern from 15-200kDa on 10% SDS-PAGE suggested that ECSAE reserves only monomeric proteins. ECSAE exhibited proteolytic activity by degrading casein. The specific activity was fond to be 0.27 units/mg/min at 37°C. The proteolytic activity was strongly inhibited by both PMSF and IAA. While, EDTA and 1,10 Phenanthroline did not show inhibition, suggesting the presence serine and cysteine protease in the seeds. ECSAE showcased procoagulant effect as reduced the clotting time of PRP from control 214.2s to 94.2s which promotes blood coagulation. ECSAE found to hydrolyze fibrin clot with specific activity of 1.1 to 4.5units in a dose dependent manner. Furthermore, ECSAE did not hydrolysis the RBC suggested its non-toxic properties.

Keywords:Elettaria Cardamomum; Protease; Antioxidant; Procoagulant